| Intrument | Microplate reader(520-540 nm,optimum wavelength: 530 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,cell culture supernatant,tissue,cells |
| Detection Principal | Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.10 mmol/L |
| Detection Range | 0.12-7.0 mmol/L |
| Recovery Rate | 105 |
| Inter CV | 3.5 |