L-Lactic Acid/Lactate (LA) Colorimetric Assay Kit

L-Lactic Acid/Lactate (LA) Colorimetric Assay Kit

Cat No.:HR3BC1206
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Microplate reader(520-540 nm,optimum wavelength: 530 nm)
Assay Time 30 min
Assay TypeQuantitative
Sample TypeSerum,plasma,cell culture supernatant,tissue,cells
Detection Principal Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated.
ReagentsNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
LabwareMicropipettor, Centrifuge, Incubator, Vortex mixer
Sensitivity 0.10 mmol/L
Detection Range 0.12-7.0 mmol/L
Recovery Rate 105
Inter CV 3.5

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