Intrument | Microplate reader(520-540 nm,optimum wavelength: 530 nm) |
Assay Time | 30 min |
Validity | 6 |
Assay Type | Quantitative |
Sample Type | Serum,plasma,cell culture supernatant,tissue,cells |
Detection Principal | Using NAD+ as H+ receptor, LDH catalyzes the reaction of lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of lactic acid can be calculated. |
Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Labware | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Size | 96T |
Sensitivity | 0.10 mmol/L |
Detection Range | 0.12-7.0 mmol/L |
Recovery Rate | 105 |
Inter CV | 3.5 |