| Intrument | Microplate reader(510-570 nm,optimum wavelength: 560 nm) |
| Assay Time | 40 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum,plasma,saliva,urine,hydrothorax,tissue |
| Synonyms | SA |
| Detection Principal | Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Labware | Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer |
| Size | 96T |
| Sensitivity | 0.03 mmol/L |
| Detection Range | 0.03-7 mmol/L |
| Recovery Rate | 95 |
| Inter CV | 6.1 |