Acid Phosphatase (ACP) Activity Assay Kit

Acid Phosphatase (ACP) Activity Assay Kit

Cat No.:HR3BC1208
Product available

  Product info

Detection Method: Colorimetric method

Storage : 2-8?
Product Details

Intrument Microplate reader(510-530 nm,optimum wavelength: 520 nm)
Assay Time 45 min
Assay TypeEnzyme Activity
Sample TypeSerum,plasma,hydrothorax,urine,cells,cell culture supernatant,animal tissue
Synonyms ACP
Detection Principal Acid phosphatase decomposes disodium phenyl phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol acts with 4-aminoantipyrine in alkaline solution, and oxidizes to a derivative of red quinone by potassium ferricyanide. The activity of the ACP can be calculated by measuring the OD value at 520 nm.
ReagentsNormal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
LabwareMicropipettor, Centrifuge, Incubator, Vortex mixer
Sensitivity 1.40 U/100 mL
Detection Range 1.40-40 U/100 mL
Recovery Rate 104
Inter CV 9.4

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