Chicken FGF2(Fibroblast Growth Factor 2, Basic) ELISA Kit


Chicken FGF2(Fibroblast Growth Factor 2, Basic) ELISA Kit

Cat No:HR1E2145
Price:$399/96T
Product available

  Product info

Alternative Name: B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 2, Basic(FGF2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibroblast Growth Factor 2, Basic(FGF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 2, Basic(FGF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Fibroblast Growth Factor 2, Basic(FGF2) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Chicken FGF2(Fibroblast Growth Factor 2, Basic) ELISA Kit
Cat NoHR1E2145
SpeciesChicken
Alternative NameB-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
Sensitivity6.3 pg/mL
Standard1000 pg/mL
Detection Range15.63-1000 pg/mL
Assay Length3.5h
Research AreaCytokine;Tumor immunity;Infection immunity;

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