Human EPO(Erythropoietin) ELISA Kit


Human EPO(Erythropoietin) ELISA Kit

Cat No:HR1E1122
Price:$399/96T
Product available

  Product info

Alternative Name: EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Erythropoietin(EPO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Erythropoietin(EPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Erythropoietin(EPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Erythropoietin(EPO) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human EPO(Erythropoietin) ELISA Kit
Cat NoHR1E1122
SpeciesHuman
Alternative NameEP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin
Sensitivity11.5 pg/mL
Standard2000 pg/mL
Detection Range31.25-2000 pg/mL
Assay Length3.5h
Research AreaCytokine;Endocrinology;Hematology;Hormone metabolism;

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