Human CFD(Complement Factor D) ELISA Kit


Human CFD(Complement Factor D) ELISA Kit

Cat No:HR1E1239
Price:$399/96T
Product available

  Product info

Alternative Name: CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor D(CFD). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Complement Factor D(CFD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor D(CFD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Complement Factor D(CFD) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human CFD(Complement Factor D) ELISA Kit
Cat NoHR1E1239
SpeciesHuman
Alternative NameCF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator
Sensitivity0.257 ng/mL
Standard40 ng/mL
Detection Range0.63-40 ng/mL
Assay Length3.5h
Research AreaImmune molecule;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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