Rat NT3(Neurotrophin 3) ELISA Kit


Rat NT3(Neurotrophin 3) ELISA Kit

Cat No:HR1E2406
Price:$399/96T
Product available

  Product info

Alternative Name: NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurotrophin 3(NT3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurotrophin 3(NT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurotrophin 3(NT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Neurotrophin 3(NT3) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat NT3(Neurotrophin 3) ELISA Kit
Cat NoHR1E2406
SpeciesRat
Alternative NameNTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor
Sensitivity1.33 pg/mL
Standard200 pg/mL
Detection Range3.13-200 pg/mL
Assay Length3.5h
Research AreaCytokine;Neuro science;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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