Rat ET-1(Endothelin1) ELISA Kit


Rat ET-1(Endothelin1) ELISA Kit

Cat No:HR1E2545
Price:$399/96T
Product available

  Product info

Alternative Name: ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Endothelin1(ET-1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Endothelin1(ET-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Endothelin1(ET-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Endothelin1(ET-1) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat ET-1(Endothelin1) ELISA Kit
Cat NoHR1E2545
SpeciesRat
Alternative NameET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1
Sensitivity2.71 pg/mL
Standard500 pg/mL
Detection Range7.82-500 pg/mL
Assay Length3.5h
Research AreaEndocrinology;Cardiovascular biology;
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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