Rat PF4(Platelet Factor 4) ELISA Kit


Rat PF4(Platelet Factor 4) ELISA Kit

Cat No:HR1E2555
Price:$399/96T
Product available

  Product info

Alternative Name: CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Factor 4(PF4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Platelet Factor 4(PF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Factor 4(PF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Platelet Factor 4(PF4) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat PF4(Platelet Factor 4) ELISA Kit
Cat NoHR1E2555
SpeciesRat
Alternative NameCXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
Sensitivity0.137 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaCytokine;Hematology;
Sample Typeserum, platelet-poor plasma and cell culture supernates

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