Rat NPY(Neuropeptide Y) ELISA Kit


Rat NPY(Neuropeptide Y) ELISA Kit

Cat No:HR1E2557
Price:$399/96T
Product available

  Product info

Alternative Name: NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide Y(NPY). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuropeptide Y(NPY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropeptide Y(NPY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Neuropeptide Y(NPY) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat NPY(Neuropeptide Y) ELISA Kit
Cat NoHR1E2557
SpeciesRat
Alternative NameNP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY
Sensitivity0.94 pg/mL
Standard200 pg/mL
Detection Range3.13-200 pg/mL
Assay Length3.5h
Research AreaMetabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hepatology;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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