Rat IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit


Rat IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit

Cat No:HR1E2610
Price:$399/96T
Product available

  Product info

Alternative Name: BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor Binding Protein 3(IGFBP3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Insulin Like Growth Factor Binding Protein 3(IGFBP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor Binding Protein 3(IGFBP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Insulin Like Growth Factor Binding Protein 3(IGFBP3) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit
Cat NoHR1E2610
SpeciesRat
Alternative NameBP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex
Sensitivity0.114 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaMetabolic pathway;Tumor immunity;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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