Rat TNFb(Tumor Necrosis Factor Beta) ELISA Kit


Rat TNFb(Tumor Necrosis Factor Beta) ELISA Kit

Cat No:HR1E2626
Price:$399/96T
Product available

  Product info

Alternative Name: LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Beta(TNFb). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tumor Necrosis Factor Beta(TNFb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Beta(TNFb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Tumor Necrosis Factor Beta(TNFb) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat TNFb(Tumor Necrosis Factor Beta) ELISA Kit
Cat NoHR1E2626
SpeciesRat
Alternative NameLT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha
Sensitivity3.3 pg/mL
Standard500 pg/mL
Detection Range7.82-500 pg/mL
Assay Length3.5h
Research AreaCytokine;Tumor immunity;Infection immunity;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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