Rat OPG(Osteoprotegerin) ELISA Kit


Rat OPG(Osteoprotegerin) ELISA Kit

Cat No:HR1E2639
Price:$399/96T
Product available

  Product info

Alternative Name: TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteoprotegerin(OPG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Osteoprotegerin(OPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteoprotegerin(OPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Osteoprotegerin(OPG) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat OPG(Osteoprotegerin) ELISA Kit
Cat NoHR1E2639
SpeciesRat
Alternative NameTNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor
Sensitivity1.21 pg/mL
Standard200 pg/mL
Detection Range3.13-200 pg/mL
Assay Length3.5h
Research AreaCytokine;Metabolic pathway;Bone metabolism;
Sample Typeserum, plasma, tissue homogenates and other biological fluids

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