Rat MMP1(Matrix Metalloproteinase 1) ELISA Kit


Rat MMP1(Matrix Metalloproteinase 1) ELISA Kit

Cat No:HR1E2651
Price:$399/96T
Product available

  Product info

Alternative Name: CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 1(MMP1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Matrix Metalloproteinase 1(MMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 1(MMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Matrix Metalloproteinase 1(MMP1) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat MMP1(Matrix Metalloproteinase 1) ELISA Kit
Cat NoHR1E2651
SpeciesRat
Alternative NameCLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase
Sensitivity0.057 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaTumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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