Rat CRH(Corticotropin Releasing Hormone) ELISA Kit


Rat CRH(Corticotropin Releasing Hormone) ELISA Kit

Cat No:HR1E2715
Price:$399/96T
Product available

  Product info

Alternative Name: CRF; Corticotropin Releasing Factor; Corticoliberin

Assay Type: Competitive Inhibition
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Corticotropin Releasing Hormone(CRH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone(CRH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Corticotropin Releasing Hormone(CRH) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat CRH(Corticotropin Releasing Hormone) ELISA Kit
Cat NoHR1E2715
SpeciesRat
Alternative NameCRF; Corticotropin Releasing Factor; Corticoliberin
Sensitivity4.95 pg/mL
Standard1000 pg/mL
Detection Range15.63-1000 pg/mL
Assay Length2h
Research AreaEndocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;Urology;
Sample Typeserum, plasma and other biological fluids

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