Guinea pig TNFa(Tumor Necrosis Factor Alpha) ELISA Kit


Guinea pig TNFa(Tumor Necrosis Factor Alpha) ELISA Kit

Cat No:HR1E1326
Price:$399/96T
Product available

  Product info

Alternative Name: TNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha( TNFa). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha( TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha( TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Tumor Necrosis Factor Alpha( TNFa) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Guinea pig TNFa(Tumor Necrosis Factor Alpha) ELISA Kit
Cat NoHR1E1326
SpeciesGuinea pig
Alternative NameTNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2
Sensitivity2.8 pg/mL
Standard500 pg/mL
Detection Range7.82-500 pg/mL
Assay Length3.5h
Research AreaCytokine;Tumor immunity;Infection immunity;

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