Mouse GCSF(Colony Stimulating Factor 3, Granulocyte) ELISA Kit


Mouse GCSF(Colony Stimulating Factor 3, Granulocyte) ELISA Kit

Cat No:HR1E1137
Product available

  Product info

Alternative Name: CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 3, Granulocyte(GCSF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Colony Stimulating Factor 3, Granulocyte(GCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 3, Granulocyte(GCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Colony Stimulating Factor 3, Granulocyte(GCSF) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse GCSF(Colony Stimulating Factor 3, Granulocyte) ELISA Kit
Cat NoHR1E1137
SpeciesMouse
Alternative NameCSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim
Sensitivity2.9 pg/mL
Standard500 pg/mL
Detection Range7.82-500 pg/mL
Assay Length3.5h
Research AreaCytokine;Hematology;

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