Human OSCP1(Organic Solute Carrier Partner 1) ELISA Kit

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Organic Solute Carrier Partner 1(OSCP1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Organic Solute Carrier Partner 1(OSCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Organic Solute Carrier Partner 1(OSCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Organic Solute Carrier Partner 1(OSCP1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Details