Goat HB(Hemoglobin) ELISA Kit

Goat HB(Hemoglobin) ELISA Kit

Cat No:HR1E4366
Product available

  Product info

Alternative Name: Hgb; Haemoglobin; Heterotetramer(??)2

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin(HB). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin(HB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hemoglobin(HB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Hemoglobin(HB) in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Details

Product Name Goat HB(Hemoglobin) ELISA Kit
Cat NoHR1E4366
Alternative NameHgb; Haemoglobin; Heterotetramer(??)2
Sensitivity2.7 ng/mL
Standard500 ng/mL
Detection Range7.82-500 ng/mL
Assay Length3.5h
Research AreaMetabolic pathway;Infection immunity;Hematology;Neuro science;

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