Mouse PICK1(Protein Interacting With Protein Kinase C Alpha 1) ELISA Kit


Mouse PICK1(Protein Interacting With Protein Kinase C Alpha 1) ELISA Kit

Cat No:HR1E4683
Price:$399/96T
Product available

  Product info

Alternative Name: PRKCABP; Protein kinase C,Alpha Binding Protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Interacting With Protein Kinase C Alpha 1(PICK1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Interacting With Protein Kinase C Alpha 1(PICK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Interacting With Protein Kinase C Alpha 1(PICK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Protein Interacting With Protein Kinase C Alpha 1(PICK1) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse PICK1(Protein Interacting With Protein Kinase C Alpha 1) ELISA Kit
Cat NoHR1E4683
SpeciesMouse
Alternative NamePRKCABP; Protein kinase C,Alpha Binding Protein; Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein
Sensitivity0.053 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaSignal transduction;Enzyme & Kinase;Metabolic pathway;
Sample TypeTissue homogenates, cell lysates and other biological fluids.

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