Human HCG(Chorionic Gonadotropin) ELISA Kit


Human HCG(Chorionic Gonadotropin) ELISA Kit

Cat No:HR1E1475
Price:$399/96T
Product available

  Product info

Alternative Name: hCG; Human Chorionic Gonadotrophin

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chorionic Gonadotropin(HCG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chorionic Gonadotropin(HCG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chorionic Gonadotropin(HCG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Chorionic Gonadotropin(HCG) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human HCG(Chorionic Gonadotropin) ELISA Kit
Cat NoHR1E1475
SpeciesHuman
Alternative NamehCG; Human Chorionic Gonadotrophin
Sensitivity0.32 ng/mL
Standard50 ng/mL
Detection Range0.79-50 ng/mL
Assay Length3.5h
Research AreaEndocrinology;Reproductive science;Neuro science;Genetic science;Hormone metabolism;
Sample Typeserum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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