Human LIPG(Lipase, Endothelial) ELISA Kit


Human LIPG(Lipase, Endothelial) ELISA Kit

Cat No:HR1E1543
Price:$399/96T
Product available

  Product info

Alternative Name: EL; EDL; Endothelial Lipase; Endothelial cell-derived lipase

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Endothelial(LIPG). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lipase, Endothelial(LIPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Endothelial(LIPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Lipase, Endothelial(LIPG) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human LIPG(Lipase, Endothelial) ELISA Kit
Cat NoHR1E1543
SpeciesHuman
Alternative NameEL; EDL; Endothelial Lipase; Endothelial cell-derived lipase
Sensitivity30 pg/mL
Standard5000 pg/mL
Detection Range78.13-5000 pg/mL
Assay Length3.5h
Research AreaEnzyme & Kinase;Metabolic pathway;
Sample TypeSerum, plasma and other biological fluids

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