Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit


Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit

Cat No:HR1E1546
Price:$399/96T
Product available

  Product info

Alternative Name: PAPP-A; PAPA; ASBABP2; DIPLA1; IGFBP-4ase; PAPPA1; Pappalysin 1; Insulin-Like Growth Factor Binding Protein-4 Protease; Differentially Placenta 1 Expressed Protein

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy Associated Plasma Protein A(PAPPA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pregnancy Associated Plasma Protein A(PAPPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnancy Associated Plasma Protein A(PAPPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Pregnancy Associated Plasma Protein A(PAPPA) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit
Cat NoHR1E1546
SpeciesHuman
Alternative NamePAPP-A; PAPA; ASBABP2; DIPLA1; IGFBP-4ase; PAPPA1; Pappalysin 1; Insulin-Like Growth Factor Binding Protein-4 Protease; Differentially Placenta 1 Expressed Protein
Sensitivity0.117 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaReproductive science;Genetic science;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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