Human AQP2(Aquaporin 2, Collecting Duct) ELISA Kit


Human AQP2(Aquaporin 2, Collecting Duct) ELISA Kit

Cat No:HR1E1557
Price:$399/96T
Product available

  Product info

Alternative Name: AQP-CD; WCH-CD; ADH water channel; Aquaporin-CD; Collecting duct water channel protein; Water channel protein for renal collecting duct

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aquaporin 2, Collecting Duct(AQP2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Aquaporin 2, Collecting Duct(AQP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aquaporin 2, Collecting Duct(AQP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Aquaporin 2, Collecting Duct(AQP2) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human AQP2(Aquaporin 2, Collecting Duct) ELISA Kit
Cat NoHR1E1557
SpeciesHuman
Alternative NameAQP-CD; WCH-CD; ADH water channel; Aquaporin-CD; Collecting duct water channel protein; Water channel protein for renal collecting duct
Sensitivity0.117 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaMetabolic pathway;Kidney biomarker;
Sample TypeTissue homogenates, urine and other biological fluids

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