Human TPO(Thyroid Peroxidase) ELISA Kit


Human TPO(Thyroid Peroxidase) ELISA Kit

Cat No:HR1E1561
Price:$399/96T
Product available

  Product info

Alternative Name: TPX; thyroperoxidase

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroid Peroxidase(TPO). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thyroid Peroxidase(TPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroid Peroxidase(TPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Thyroid Peroxidase(TPO) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human TPO(Thyroid Peroxidase) ELISA Kit
Cat NoHR1E1561
SpeciesHuman
Alternative NameTPX; thyroperoxidase
Sensitivity0.112 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaEnzyme & Kinase;Endocrinology;Hormone metabolism;
Sample TypeTissue homogenates and other biological fluids.

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