Human ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit


Human ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit

Cat No:HR1E1566
Price:$399/96T
Product available

  Product info

Alternative Name: TRAP; TrATPase; Tartrate Resistant Acid Phosphatase; Type 5 acid phosphatase; TRACP 5; tartrate-resistant acid phosphatase 5b

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acid Phosphatase 5, Tartrate Resistant(ACP5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Acid Phosphatase 5, Tartrate Resistant(ACP5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acid Phosphatase 5, Tartrate Resistant(ACP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Acid Phosphatase 5, Tartrate Resistant(ACP5) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human ACP5(Acid Phosphatase 5, Tartrate Resistant) ELISA Kit
Cat NoHR1E1566
SpeciesHuman
Alternative NameTRAP; TrATPase; Tartrate Resistant Acid Phosphatase; Type 5 acid phosphatase; TRACP 5; tartrate-resistant acid phosphatase 5b
Sensitivity0.129 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaEnzyme & Kinase;Metabolic pathway;Hepatology;
Sample TypeSerum, plasma, tissue homogenates and other biological fluids

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