Human HSPb2(Heat Shock Protein Beta 2) ELISA Kit


Human HSPb2(Heat Shock Protein Beta 2) ELISA Kit

Cat No:HR1E1600
Price:$399/96T
Product available

  Product info

Alternative Name: HSP-B2; MKBP; HSP27; Heat Shock 27kDa Protein 2; DMPK-binding protein

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein Beta 2(HSPb2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein Beta 2(HSPb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein Beta 2(HSPb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Heat Shock Protein Beta 2(HSPb2) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human HSPb2(Heat Shock Protein Beta 2) ELISA Kit
Cat NoHR1E1600
SpeciesHuman
Alternative NameHSP-B2; MKBP; HSP27; Heat Shock 27kDa Protein 2; DMPK-binding protein
Sensitivity0.265 ng/mL
Standard40 ng/mL
Detection Range0.63-40 ng/mL
Assay Length3.5h
Research AreaSignal transduction;Tumor immunity;Developmental science;
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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