Human HSP40(Heat Shock Protein 40) ELISA Kit


Human HSP40(Heat Shock Protein 40) ELISA Kit

Cat No:HR1E1601
Price:$399/96T
Product available

  Product info

Alternative Name: DnaJ; DNAJB1; Sis1; RSPH16B; HSPF1; DnaJ homolog subfamily B member 1; Radial Spoke 16 Homolog B; Heat Shock 40kDa Protein 1; DnaJ protein homolog 1

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 40(HSP40). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein 40(HSP40). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 40(HSP40), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Heat Shock Protein 40(HSP40) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human HSP40(Heat Shock Protein 40) ELISA Kit
Cat NoHR1E1601
SpeciesHuman
Alternative NameDnaJ; DNAJB1; Sis1; RSPH16B; HSPF1; DnaJ homolog subfamily B member 1; Radial Spoke 16 Homolog B; Heat Shock 40kDa Protein 1; DnaJ protein homolog 1
Sensitivity0.054 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaSignal transduction;Developmental science;
Sample Typetissue homogenates, cell lysates and other biological fluids

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