Human NOS3(Nitric Oxide Synthase 3, Endothelial) ELISA Kit


Human NOS3(Nitric Oxide Synthase 3, Endothelial) ELISA Kit

Cat No:HR1E1610
Price:$399/96T
Product available

  Product info

Alternative Name: ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 3, Endothelial(NOS3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nitric Oxide Synthase 3, Endothelial(NOS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 3, Endothelial(NOS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Nitric Oxide Synthase 3, Endothelial(NOS3) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human NOS3(Nitric Oxide Synthase 3, Endothelial) ELISA Kit
Cat NoHR1E1610
SpeciesHuman
Alternative NameECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS
Sensitivity0.059 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaEnzyme & Kinase;Cardiovascular biology;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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