Dog F8(Coagulation Factor VIII) ELISA Kit


Dog F8(Coagulation Factor VIII) ELISA Kit

Cat No:HR1E6114
Price:$399/96T
Product available

  Product info

Alternative Name: FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII(F8). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coagulation Factor VIII(F8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VIII(F8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Coagulation Factor VIII(F8) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Dog F8(Coagulation Factor VIII) ELISA Kit
Cat NoHR1E6114
SpeciesDog
Alternative NameFVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein
Sensitivity28 pg/mL
Standard5000 pg/mL
Detection Range78.13-5000 pg/mL
Assay Length3.5h
Research AreaMetabolic pathway;Hematology;
Sample Typeplasma

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