Mouse UQCRC2(Ubiquinol Cytochrome C Reductase Core Protein II) ELISA Kit

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquinol Cytochrome C Reductase Core Protein II(UQCRC2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ubiquinol Cytochrome C Reductase Core Protein II(UQCRC2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquinol Cytochrome C Reductase Core Protein II(UQCRC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Ubiquinol Cytochrome C Reductase Core Protein II(UQCRC2) in the samples is then determined by comparing the OD of the samples to the standard curve.

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