Human MIF(Macrophage Migration Inhibitory Factor) ELISA Kit


Human MIF(Macrophage Migration Inhibitory Factor) ELISA Kit

Cat No:HR1E1616
Price:$399/96T
Product available

  Product info

Alternative Name: GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Migration Inhibitory Factor(MIF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Migration Inhibitory Factor(MIF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Migration Inhibitory Factor(MIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Macrophage Migration Inhibitory Factor(MIF) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human MIF(Macrophage Migration Inhibitory Factor) ELISA Kit
Cat NoHR1E1616
SpeciesHuman
Alternative NameGIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase
Sensitivity0.128 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaCytokine;Infection immunity;Rheumatology;Autoimmunity;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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