Human Bax(Bcl2 Associated X Protein) ELISA Kit


Human Bax(Bcl2 Associated X Protein) ELISA Kit

Cat No:HR1E1642
Price:$399/96T
Product available

  Product info

Alternative Name: BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated X Protein(Bax). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Bcl2 Associated X Protein(Bax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated X Protein(Bax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Bcl2 Associated X Protein(Bax) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human Bax(Bcl2 Associated X Protein) ELISA Kit
Cat NoHR1E1642
SpeciesHuman
Alternative NameBCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4
Sensitivity0.3 ng/mL
Standard50 ng/mL
Detection Range0.79-50 ng/mL
Assay Length3.5h
Research AreaSignal transduction;Apoptosis;
Sample Typeserum, plasma, tissue homogenates, cell lysates and other biological fluids

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