Human COPRS(Coordinator Of PRMT5 And Differentiation Stimulator) ELISA Kit

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coordinator Of PRMT5 And Differentiation Stimulator(COPRS). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Coordinator Of PRMT5 And Differentiation Stimulator(COPRS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coordinator Of PRMT5 And Differentiation Stimulator(COPRS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Coordinator Of PRMT5 And Differentiation Stimulator(COPRS) in the samples is then determined by comparing the OD of the samples to the standard curve.

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