Chicken IFNg(Interferon Gamma) ELISA Kit


Chicken IFNg(Interferon Gamma) ELISA Kit

Cat No:HR1E1718
Price:$399/96T
Product available

  Product info

Alternative Name: IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Gamma(IFNg). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon Gamma(IFNg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Gamma(IFNg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Interferon Gamma(IFNg) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Chicken IFNg(Interferon Gamma) ELISA Kit
Cat NoHR1E1718
SpeciesChicken
Alternative NameIFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon
Sensitivity6.1 pg/mL
Standard1000 pg/mL
Detection Range15.63-1000 pg/mL
Assay Length3.5h
Research AreaCytokine;Infection immunity;

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