Cattle VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit


Cattle VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit

Cat No:HR1E7796
Price:$399/96T
Product available

  Product info

Alternative Name: VEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor A(Cattle VEGFA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vascular Endothelial Growth Factor A(Cattle VEGFA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vascular Endothelial Growth Factor A(Cattle VEGFA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Vascular Endothelial Growth Factor A(Cattle VEGFA) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Cattle VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
Cat NoHR1E7796
SpeciesCattle
Alternative NameVEGF; VEGF-A; VPF; Vascular Endothelial Cell Growth Factor A; Vascular Permeability Factor
Sensitivity5.9 pg/mL
Standard1000 pg/mL
Detection Range15.63-1000 pg/mL
Assay Length3.5h
Research AreaCytokine;Apoptosis;Tumor immunity;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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