Human MIEN1(Migration And Invasion Enhancer 1) ELISA Kit

Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Migration And Invasion Enhancer 1(MIEN1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Migration And Invasion Enhancer 1(MIEN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Migration And Invasion Enhancer 1(MIEN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Migration And Invasion Enhancer 1(MIEN1) in the samples is then determined by comparing the OD of the samples to the standard curve.

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