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General HVD3(25-Hydroxyvitamin D3) ELISA Kit


General HVD3(25-Hydroxyvitamin D3) ELISA Kit

Cat No:HR1E7962
Price:$399/96T
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Alternative Name: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
Assay Type: Competitive Inhibition
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 25-Hydroxyvitamin D3(HVD3) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 25-Hydroxyvitamin D3(HVD3). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of 25-Hydroxyvitamin D3(HVD3) in the samples is then determined by comparing the OD of the samples to the standard curve.


Product Details
Product Name General HVD3(25-Hydroxyvitamin D3) ELISA Kit
Cat NoHR1E7962
SpeciesGeneral
Alternative Name25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
Sensitivity0.91 ng/mL
Standard200 ng/mL
Detection Range3.13-200 ng/mL
Assay Length2h
Research AreaReproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Sample Typeserum, plasma and other biological fluids

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