Mouse KEAP1(Kelch Like ECH Associated Protein 1) ELISA Kit


Mouse KEAP1(Kelch Like ECH Associated Protein 1) ELISA Kit

Cat No:HR1E8177
Price:$399/96T
Product available

  Product info

Alternative Name: INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kelch Like ECH Associated Protein 1(KEAP1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kelch Like ECH Associated Protein 1(KEAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kelch Like ECH Associated Protein 1(KEAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Kelch Like ECH Associated Protein 1(KEAP1) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse KEAP1(Kelch Like ECH Associated Protein 1) ELISA Kit
Cat NoHR1E8177
SpeciesMouse
Alternative NameINrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19
Sensitivity0.055 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaSignal transduction;
Sample Typetissue homogenates, cell lysates and other biological fluids

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