Mouse RSPO2(R-Spondin 2) ELISA Kit


Mouse RSPO2(R-Spondin 2) ELISA Kit

Cat No:HR1E8222
Price:$399/96T
Product available

  Product info

Alternative Name: Roof plate-specific spondin-2

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 2(RSPO2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to R-Spondin 2(RSPO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 2(RSPO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of R-Spondin 2(RSPO2) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse RSPO2(R-Spondin 2) ELISA Kit
Cat NoHR1E8222
SpeciesMouse
Alternative NameRoof plate-specific spondin-2
Sensitivity29 pg/mL
Standard5000 pg/mL
Detection Range78.13-5000 pg/mL
Assay Length3.5h
Research AreaSignal transduction;
Sample Typeserum, plasma, tissue homogenates and other biological fluids

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