Mouse ATP1b4(ATPase, Na+/K+ Transporting Beta 4 Polypeptide) ELISA Kit


Mouse ATP1b4(ATPase, Na+/K+ Transporting Beta 4 Polypeptide) ELISA Kit

Cat No:HR1E8226
Price:$399/96T
Product available

  Product info

Alternative Name: X,K-ATPase subunit beta-m; X/potassium-transporting ATPase subunit beta-m

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide(ATP1b4). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATPase, Na+/K+ Transporting Beta 4 Polypeptide(ATP1b4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, Na+/K+ Transporting Beta 4 Polypeptide(ATP1b4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of ATPase, Na+/K+ Transporting Beta 4 Polypeptide(ATP1b4) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse ATP1b4(ATPase, Na+/K+ Transporting Beta 4 Polypeptide) ELISA Kit
Cat NoHR1E8226
SpeciesMouse
Alternative NameX,K-ATPase subunit beta-m; X/potassium-transporting ATPase subunit beta-m
Sensitivity0.057 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaEnzyme & Kinase;
Sample TypeTissue homogenates, cell lysates and other biological fluids

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