Mouse CYCS(Cytochrome C, Somatic) ELISA Kit


Mouse CYCS(Cytochrome C, Somatic) ELISA Kit

Cat No:HR1E8288
Price:$399/96T
Product available

  Product info

Alternative Name: Cyt-C; HCS; CYC; CytC

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C, Somatic(CYCS). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome C, Somatic(CYCS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C, Somatic(CYCS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Cytochrome C, Somatic(CYCS) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse CYCS(Cytochrome C, Somatic) ELISA Kit
Cat NoHR1E8288
SpeciesMouse
Alternative NameCyt-C; HCS; CYC; CytC
Sensitivity0.122 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaMetabolic pathway;Developmental science;
Sample Typetissue homogenates, cell lysates, cell culture supernates and other biological fluids

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