Mouse very low density lipoprotein(VLDL) ELISA Kit


Mouse very low density lipoprotein(VLDL) ELISA Kit

Cat No:HR1E8379
Price:$399/96T
Product available

  Product info

Alternative Name: Pre-?-Lipoprotein; Pre-Beta Lipoprotein

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VLDL(very low density lipoprotein). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VLDL(very low density lipoprotein). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VLDL(very low density lipoprotein), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of VLDL(very low density lipoprotein) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse very low density lipoprotein(VLDL) ELISA Kit
Cat NoHR1E8379
SpeciesMouse
Alternative NamePre-?-Lipoprotein; Pre-Beta Lipoprotein
Sensitivity0.195 ng/mL
Standard50 ng/mL
Detection Range0.79-50 ng/mL
Assay Length3.5h
Research Areaserum, plasma and other biological fluids
Sample Typeserum, plasma, cell lysates, cell culture supernates and other biological fluids

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