Rat TLR3(Toll Like Receptor 3) ELISA Kit


Rat TLR3(Toll Like Receptor 3) ELISA Kit

Cat No:HR1E8413
Price:$399/96T
Product available

  Product info

Alternative Name: CD283

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll Like Receptor 3(TLR3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Toll Like Receptor 3(TLR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Toll Like Receptor 3(TLR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Toll Like Receptor 3(TLR3) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Rat TLR3(Toll Like Receptor 3) ELISA Kit
Cat NoHR1E8413
SpeciesRat
Alternative NameCD283
Sensitivity0.055 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaSignal transduction;Tumor immunity;Infection immunity;Immune molecule;Pulmonology;
Sample Typetissue homogenates, cell lysates and other biological fluids

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