Mouse PYCARD(PYD And CARD Domain Containing Protein) ELISA Kit


Mouse PYCARD(PYD And CARD Domain Containing Protein) ELISA Kit

Cat No:HR1E8468
Price:$399/96T
Product available

  Product info

Alternative Name: ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PYD And CARD Domain Containing Protein(PYCARD). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PYD And CARD Domain Containing Protein(PYCARD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PYD And CARD Domain Containing Protein(PYCARD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of PYD And CARD Domain Containing Protein(PYCARD) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse PYCARD(PYD And CARD Domain Containing Protein) ELISA Kit
Cat NoHR1E8468
SpeciesMouse
Alternative NameASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1
Sensitivity3 pg/mL
Standard500 pg/mL
Detection Range7.82-500 pg/mL
Assay Length3.5h
Research AreaApoptosis;
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

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