Mouse NFKB-p65(NuclearFactor Kappa B p65) ELISA Kit


Mouse NFKB-p65(NuclearFactor Kappa B p65) ELISA Kit

Cat No:HR1E8472
Price:$399/96T
Product available

  Product info

Alternative Name: NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NuclearFactor Kappa B p65(NFKB-p65). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NuclearFactor Kappa B p65(NFKB-p65). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NuclearFactor Kappa B p65(NFKB-p65), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of NuclearFactor Kappa B p65(NFKB-p65) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse NFKB-p65(NuclearFactor Kappa B p65) ELISA Kit
Cat NoHR1E8472
SpeciesMouse
Alternative NameNFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A
Sensitivity11.2 pg/mL
Standard2000 pg/mL
Detection Range31.25-2000 pg/mL
Assay Length3.5h
Research AreaApoptosis;
Sample TypeTissue homogenates, cell lysates and other biological fluids

Write us

Superfast Delivery
Customer Support
Secure Online Payment