Mouse TRAF6(TNF Receptor Associated Factor 6) ELISA Kit


Mouse TRAF6(TNF Receptor Associated Factor 6) ELISA Kit

Cat No:HR1E8478
Price:$399/96T
Product available

  Product info

Alternative Name: RNF85; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 6(TRAF6). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 6(TRAF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 6(TRAF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of TNF Receptor Associated Factor 6(TRAF6) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse TRAF6(TNF Receptor Associated Factor 6) ELISA Kit
Cat NoHR1E8478
SpeciesMouse
Alternative NameRNF85; E3 ubiquitin-protein ligase TRAF6; Interleukin-1 signal transducer
Sensitivity0.053 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaTumor immunity;
Sample TypeTissue homogenates, cell lysates and other biological fluids.

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