Human UQCRFS1(Ubiquinol-cytochrome c reductase) ELISA Kit


Human UQCRFS1(Ubiquinol-cytochrome c reductase) ELISA Kit

Cat No:HR1E8542
Price:$399/96T
Product available

  Product info

Alternative Name: Complex III subunit 5; Complex III subunit IX; Rieske iron sulfur protein; RIP1; RIS1; RISP; UQCR5; UQCRFS1

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquinol-cytochrome c reductase(UQCRFS1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ubiquinol-cytochrome c reductase(UQCRFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquinol-cytochrome c reductase(UQCRFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Ubiquinol-cytochrome c reductase(UQCRFS1) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human UQCRFS1(Ubiquinol-cytochrome c reductase) ELISA Kit
Cat NoHR1E8542
SpeciesHuman
Alternative NameComplex III subunit 5; Complex III subunit IX; Rieske iron sulfur protein; RIP1; RIS1; RISP; UQCR5; UQCRFS1
Sensitivity0.119 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaOxidative phosphorylation, metabolic pathways

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