Mouse GC(Glucocorticoid) ELISA Kit


Mouse GC(Glucocorticoid) ELISA Kit

Cat No:HR1E8561
Price:$399/96T
Product available

  Product info

Alternative Name: Glucocorticoid; GC

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucocorticoid(GC). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucocorticoid(GC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucocorticoid(GC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Glucocorticoid(GC) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Mouse GC(Glucocorticoid) ELISA Kit
Cat NoHR1E8561
SpeciesMouse
Alternative NameGlucocorticoid; GC
Sensitivity0.118 ng/mL
Standard20 ng/mL
Detection Range0.32-20 ng/mL
Assay Length3.5h
Research AreaSignal transduction;Metabolic pathway;Endocrinology;Hormone metabolism;
Sample Typetissue homogenates, cell lysates and other biological fluids

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