Human APAF1(Apoptotic Peptidase Activating Factor 1) ELISA Kit


Human APAF1(Apoptotic Peptidase Activating Factor 1) ELISA Kit

Cat No:HR1E1859
Price:$399/96T
Product available

  Product info

Alternative Name: CED4; Apoptotic Protease Activating Factor

Assay Type: Sandwich
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1(APAF1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1(APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apoptotic Peptidase Activating Factor 1(APAF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ? 10nm. The concentration of Apoptotic Peptidase Activating Factor 1(APAF1) in the samples is then determined by comparing the OD of the samples to the standard curve.



Product Details

Product Name Human APAF1(Apoptotic Peptidase Activating Factor 1) ELISA Kit
Cat NoHR1E1859
SpeciesHuman
Alternative NameCED4; Apoptotic Protease Activating Factor
Sensitivity0.057 ng/mL
Standard10 ng/mL
Detection Range0.16-10 ng/mL
Assay Length3.5h
Research AreaApoptosis;

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